Aflatoxin B1(AFB1) is a potent hepatotoxic and hepatocarcinogenic mycotoxin in human beings. It is accumulated in animal tissues and injured cell through variable metabolic pathway. This study was conducted to determine the
effect
of antioxidant vitamins on liver function enzymes and hepatic damage of AFB1 treated mice. The 6 weeks old male ICR mice were radnomly separated 6 groups, vehicle solvent or vitamin C(10 ㎎/㎏/day) and vitamin E(63.8 ㎎/㎏/day) were
administered by interaperitoneal(i.p.) injection and 1 hr later, vehicle solution(DMSO) or AFB1(0.4 ㎎/㎏) were injected. The results obtained as follow ; The levels of liver function enzymes such as GOT, GPT, LDH, and alkaline
phosphatase,
in sera of mice were remarkably elevated by treatment with AFB1 only. However, those enzymes were significantly alleviated by co-treatment with antioxidant vitamins(p<0.01). Especially the levels of LDH and ALK phosphatase were similar
to
those of control groups(p<0.01). The transmission electron microscopy(TEM) image of intracellular microrganelles on the liver cell of mice was also degenerated extremely by treatment with AFB1, but vitamin C and vitamin E gave good
effects on
cellular deformation. The intracellular microrganelles such as mitochondria, endoplasmic reticulum, nucleus and nucleic membrane were nearly disappeared the cellular deformation by antioxidant vitamins co-administration. With above results, we
could
estimated that antioxidant vitamins blocked AFB1 induced hepatic cell damage.
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